G
Genetic Archaeology
GENETIC ARCHAEOLOGY // PROFILE

NOD2

Nucleotide Binding Oligomerization Domain Containing 2

CHR 16
16q12.1

Overview

NOD2 (also called CARD15) is an important protein of the innate immune system. It acts as an intracellular detector for bacterial cell wall components. Especially in the small intestine, it is crucial for detecting harmful bacteria and maintaining the integrity of the mucosa.

📍 Chromosomal Position

16q12.1 (Chromosome 16)

🧬 Gene Category

Immune System

🔬 Inheritance

Complex / Multifactorial

📊 Prevalence

Variants present in approx. 10-15% of the population

Function & Significance

NOD2 recognizes muramyl dipeptide (MDP), an element of the bacterial cell wall. Once NOD2 binds MDP, it activates signaling pathways (such as NF-kB) that initiate inflammatory processes and trigger the release of antibacterial substances. A defect in this system allows bacteria to cross the intestinal barrier more easily.

⚠️ Crohn’s Disease Risk

NOD2 was the first gene clearly associated with Crohn’s disease, a chronic inflammatory bowel disease (IBD). Paradoxically, mutations often lead to a *diminished* initial immune response to intestinal bacteria, which later results in excessive, chronic inflammation.

🧬 Relevant SNPs

Three main mutations in the NOD2 gene are responsible for the increased risk of Crohn’s disease:

rs2066844
16:50693581
Allele 1 (C)

Arg

/

Allele 2 (T)

Trp

Arg702Trp:
This variant impairs bacterial recognition. Carriers have an approx. 2- to 4-fold increased risk of Crohn’s disease.

rs2812378
16:50700141
Allele 1 (C)

Normal

/

Allele 2 (T)

Loss

1007fs (Leu1007fsTer2):
Leads to a shortened, non-functional NOD2 protein. This is the strongest known risk variant for Crohn’s disease (up to 15-fold risk if homozygous).

📚 Data Sources

  • OMIM: #605956 – Nucleotide Binding Oligomerization Domain Containing 2; NOD2
  • dbSNP: rs2066844, rs2812378, rs2066845
  • ClinVar: Pathogenic variants in IBD

Biological Function

The NOD2 protein recognizes bacterial cell wall components and activates the immune response. It is particularly important for maintaining the gut barrier and microbiome balance.

Associated Conditions

Crohn's Disease Blau Syndrome Graft-versus-Host Reaction Sarcoidosis
Molecular Analysis

Analyzed Markers

rs2066844 Risk Factor
Pos: 16:50693581 | Alleles: C/T

Arg702Trp - One of the three main variants significantly increasing Crohn's Disease risk (approx. 2-4 fold in heterozygotes).

rs2812378 Pathogenic
Pos: 16:50700141 | Alleles: C/T

Leu1007fsTer2 - Leads to a truncated protein. The strongest risk variant for Crohn's Disease.